The soundtrack for iota borrows from some of the most well known pieces of classical music and filters them through electronic instruments with some sampling of well known drum breaks for the rhythm section.

The main menu theme is an interpretation of Adagio by Bach.

The first level uses the opening piano phrase from Debussy's Reverie as a building block.

The second level is a mash of Gymnopedie by Erik Satie and Ave Maria.

The third level is Cello Suite No 1 by Bach and Ode to Joy by Beethoven.

The final level is Sospiro by Franz Liszt and The Swan by Camille Saint-Saens.